emsa probe biotin labeling kit (Beyotime)
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emsa probe biotin labeling kit
Emsa Probe Biotin Labeling Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/emsa+probe+biotin+labeling+kit/emsa+probe+biotin+labeling+kit/pmc12261083-246-10-15
Average 90 stars, based on 1 article reviews
Emsa Probe Biotin Labeling Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/emsa+probe+biotin+labeling+kit/emsa+probe+biotin+labeling+kit/pmc12261083-246-10-15
Average 90 stars, based on 1 article reviews
emsa probe biotin labeling kit - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Two DNA-binding One Zinc Finger transcription factors, MdCDOF3 and MdDOF3.6, accelerate leaf senescence by activating cytokinin oxidase MdCKX7 in response to sorbitol signaling in apple"
Article Title: Two DNA-binding One Zinc Finger transcription factors, MdCDOF3 and MdDOF3.6, accelerate leaf senescence by activating cytokinin oxidase MdCKX7 in response to sorbitol signaling in apple
Journal: Horticulture Research
doi: 10.1093/hr/uhaf120
Figure Legend Snippet: MdCDOF3 and MdDOF3.6 bind to MdCKX7 promoter and activate its transcription. (A) Yeast one-hybridization (Y1H) assays showed that MdCDOF3 and MdDOF3.6 interacted with MdCKX7 promoter. The 2200 bp upstream MdCKX7 promoter sequence of ATG is used for detection. (B-C) The relative levels of MdCKX7 promoter fragment enriched by MdCDOF3 (B) and MdDOF3.6 (C) detected by chromatin immunoprecipitation (ChIP)-qPCR. Data are expressed as the mean ± SEM of three biological replicates. Different letters (a and b) indicate significant differences ( P < 0.05) using Duncan’s multiple range test (MRT) after ANOVA. (D–E) Electrophoretic shift assay (EMSA) determination of the interaction of MdCDOF3 (D) and MdDOF3.6 (E) with labeled DNA Probes in the MdCKX7 promoter. (F-I) Luciferase assays showed that MdCDOF3 (F and G) and MdDOF3.6 (H and I) activated MdCKX7 expression in Tobacco leaves by transient injection. Data are expressed as the mean ± SEM of three biological replicates. Different letters (a, b, and c) indicate significant differences ( P < 0.05) using Duncan’s multiple range test (MRT) after ANOVA. (J-K) GUS staining and activity of apple calli co-expressing different combinations of recombinant plasmids including MdCKX7 pro ::GUS, 35S pro ::MdCDOF3 + MdCKX7 pro ::GUS, and 35S pro ::MdDOF3.6 + MdCKX7 pro ::GUS. Data are expressed as the mean ± SEM of three biological replicates. Different letters (a, b and c) indicate significant differences ( P < 0.05) using Duncan’s multiple range test (MRT) after ANOVA.
Techniques Used: Hybridization, Sequencing, Chromatin Immunoprecipitation, ChIP-qPCR, Shift Assay, Labeling, Luciferase, Expressing, Injection, Staining, Activity Assay, Recombinant
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